Journal: Cancers

Article Title: CD83, a Novel MAPK Signaling Pathway Interactor, Determines Ovarian Cancer Cell Fate

doi: 10.3390/cancers12082269

Figure Lengend Snippet: Integrated transcriptome and proteome analyses. ( a ) Volcano plots show the differentially expressed genes of NC-vs.-KD and NC-vs.-OV, as revealed by transcriptome. ( b ) CD83-KD SKOV3 cells exhibited elevated expression of matrix metallopeptidases (MMPs) and TGF-beta family members as compared with NC and CD83-OV cells. ( c ) The expression of cell cycle regulators and stemness-related genes was upregulated in CD83-OV SKOV3 cells. ( d ) The intersection of the transcriptome (fold change ≥2) and proteome (fold change ≥1.5) identified 83 DE proteins (and protein-coding genes) between CD83-KD and NC SKOV3 cells. Correlation analysis of DE genes and DE proteins indicated the consistency (R = 0.5360) between transcriptome and proteome in this study. ( e ) Venn diagram shows the downregulated proteins (e.g., CD83, KIT) and upregulated proteins (e.g., MMP1, MMP7) in the CD83-KD SKOV3 cells. ( f ) Content of MMP1 and MMP7 in SKOV3 cell lysate and culture media by ELISA assay. The differences between KD and NC, as well as KD and OV, were presented as the mean±SEM; Student’s t -test; ** p < 0.01.

Article Snippet: MMP1 and MMP7 concentrations from cell lysate and cell culture supernatant were measured using MMP1 ELISA Kit (Abcam, Cambridge, MA, USA) and MMP7 ELISA Kit (CUSABIO, Wuhan, China), respectively, following the manufacturer’s instructions.

Techniques: Expressing, Enzyme-linked Immunosorbent Assay